Note: Since this post was published I have done many further spectroscopy projects which I recommend checking out such as making a low-cost colourimeter for water quality sensing, fluorimeter for oil detection and a laser cut spectrometer which vastly outperforms the spectroscope shown in this post.
Science
Spectrophotometer
As light passes through a liquid it can be absorbed by chemicals found in the liquid. Different molecules will absorb at different wavelengths.
Figure 1 | Diagram for absorbance in a cuvette of solution by CarlosRC
As the light travels through the liquid it drops off exponentially with distance. The intensity of light out I1 is then dependent exponentially with the width of the cuvette. The transmittance is then given by the intensity out, over the intensity in.
$\displaystyle T=I_{1}/I_{0}$
In order to get a linear relationship absorbance is defined as the logarithm of the transmittance. This gives a linear relationship.
$\displaystyle -log_{10}T=A=-log_{10}(I_{1}/I_{0})$
The absorbance has been found to be related to the number of molecules and their cross sectional area. This can then be found The relationship between absorbance, concentration, pathlength and molar absorptivity is called the Beer-Lambert Law.
$A=\epsilon L c$
Using this equation if you know the molar absorptivity e, path length of the cuvette l you can work out the concentration c from the absorbance of light A. This is used by almost all chemistry and biology laboratories to work out concentration of solutions.
Spectrometer
A spectrometer works by splitting light made of many wavelengths (e.g. white light) into individual rays that can be detected. This allows the spectrometer to be able to find the absorbance of different wavelengths and determine what molecules are in the solution.
The basic idea of splitting light is to change the angle of the light in order to spread the colors over a large area so they can be detected separate from each other. In this project a CD was used as a grating. A grating works at separating out colors by the different path lengths between different rays reflecting and interfering like water waves can constructively and destructively interfere. This leads to different wavelengths of light being reflected at different angles. This can be calculated from Snell's law and some simple optics leads to
$d\left(\sin \theta_m+\sin \theta_i\right)=m\lambda$
The distance between the grooves is 1.6 micrometre. You can calculate the angle of refraction when;
d is the spacing between the grating
m is an integer and theta m is the angle
theta i is the incoming (incident) angle
and lambda is the wavelength of light
Design
I used a great program made by Alexander Scheeline and Kathleen Kelly from the University of Illinois who put together the Cell Phone Spectrometer website with a program that takes two pictures from a cell phone of a spectrum, a reference and a sample. Giving an absorbance reading that can be exported as comma separated values (.csv) file that can be imported into a plotting program.
Figure 6 | Cell phone spectrometer program using two pictures taken with the webcam.
To test the spectrometer a pink sample of Purpurin in methanol which is an anthraquinone was used. Anthraquinone's have been used as a dye in many paintings. In the lab were I am working a student is doing a PhD project trying to understand how these molecules break down in order to be able to aid in art restoration. The absorption spectrum was found using a halogen light source and an Ocean Optics USB-4000 mini spectrometer. Both spectrometers where referenced with methanol and compared in the following graph.
Many different designs were combined. The spectrometer was designed with the CD grating which has been used on many different DIY spectroscopes and often used with digital cameras. Also spectrophotometers have been made that use light dependent resistors (LDR) and LED's of different colours.
This design combines the spectrophotometer with a light dependent resistor and LED.As well as a spectrometer using a CD as a grating to split the light and a webcam. My thanks goes out to all the great spectrometer designs people have shared on the web.
Spectrophotometer
There are two white LED's. The top LED is detected via the light dependent resistor LDR behind the cuvette. A PICAXE 08M micro controller was used as a simple data logger collecting the absorbance data through its inbuilt 10 bit (2^10 = 1024 different possible data values) analog to digital convertor. This is then sent to the computer as an ASCI string which can be collected using a simple serial terminal such as real term.
The code for the PICAXE is a simple basic like language which is incredibly easy to code.
Main:
high C.4
readadc10 C.2, w0
sertxd (#w0)
pause 500
goto main
The data is then transferred via the download cable at 4800,n,8,1 baud rate to the computer and can be saved into a spreadsheet to do time resolved measurements.
Figure 2 | Spectrophotometer showing the battery pack and the two white LED light sources.
Spectrometer
Another LED was placed at the base of the cuvette. The cuvette has a slit created by two pieces of black insulation tape. The light is then split using a CD grating and detected by a logitech webcam. The webcam has an adjustable focus that can focus almost up to the lens itself. However the optics are not ideal as only around 200 pixels are detecting the strip of color. I took the IR filter off of the lens so it should be capable of imaging the near IR part of the spectrum.
Figure 3 | Spectrometer showing the webcam and CD diffraction grating.
Figure 4 and 5 | Spectrometer and prototyping board with cuvette holder.
I used a great program made by Alexander Scheeline and Kathleen Kelly from the University of Illinois who put together the Cell Phone Spectrometer website with a program that takes two pictures from a cell phone of a spectrum, a reference and a sample. Giving an absorbance reading that can be exported as comma separated values (.csv) file that can be imported into a plotting program.
Testing
To test the spectrometer a pink sample of Purpurin in methanol which is an anthraquinone was used. Anthraquinone's have been used as a dye in many paintings. In the lab were I am working a student is doing a PhD project trying to understand how these molecules break down in order to be able to aid in art restoration. The absorption spectrum was found using a halogen light source and an Ocean Optics USB-4000 mini spectrometer. Both spectrometers where referenced with methanol and compared in the following graph.
Figure 7 | Absorbance of Purpurin for the homemade spectrometer compared with a commercial spectrometer.
The homemade spectrometer doesn't have very good resolution at around ~3 nm the commercial spectrometer has a resolution of 0.3 nm. The homemade device seems to replicate the shape of the peak of the purpurin centered at ~500 nm. However the noise is quite bad which can be seen to be overlaid on the curve which is the main source of error in the absorbance. This could possibly be reduced by taking many pictures and collecting an average which is what the commercial unit does to reduce noise.
The spectrophotometer with the LDR and LED was not tested but I plan to test it with an oscillating reaction as well as some simple color changing reaction to teach basic kinetics. I am also thinking of adding multiple LED's in order to measure absorbance at various wavelengths.
I am also planning to make up a proper pcb board that can be used to mount the electronics in a more reliable way. I also need to improve the optics to fill the camera with the spectrum as currently only a small fraction of the CCD is receiving the spectrum.
But for around 50 dollars NZ the spectrometer performed pretty well compared with the 3,000 dollar NZ, Ocean Optics commercial spectrometer.
Links
Links
USB-spectrophotometer
Arduino controller spectrophotometer
Digital camera transmission grating spectrometer
Digital camera spectrometer
Simple LED LDR spectrophotometer
Cell phone spectrometer (with some great software)Cereal box spectroscope
Arduino controller spectrophotometer
Digital camera transmission grating spectrometer
Digital camera spectrometer
Simple LED LDR spectrophotometer
Cell phone spectrometer (with some great software)Cereal box spectroscope
To find out more about the science have a look at these links which give you a good summary and good links as well to other websites.
